![]() CULTURE OF SALMONELLA TYPHI ON BLOOD AGAR MEDIUM no hemolysis occur on blood agar medium, the colonies are grayish white and grows well. In Blood Agar medium, the Salmonella typhi shows gamma hemolysis i.e. In liquid culture media like Trypticase soy broth or Nutrient broth, the growth of the bacterium occurs as turbidity in the broth medium but the chances of contamination are high especially when the isolation is carried out from Feces specimen, commonly the Selenite F broth and Tetrathionate broth is used for the isolation of Salmonella typhi. PREPARATION OF MUELLER HINTON AGAR MEDIA (MHA) IN LABORATORY Xylose Lysine Deoxycholate Agar (XLD) MediumĬheck out the Preparation of Mueller Hinton Agar (MHA) medium for Antibiotic sensitivity test (AST) typhi contains a Brilliant green dye which is inhibitory to many Gram-positive organisms and Bismuth sulfite which is inhibitory to many gram-negative enteric organisms and supports the growth of Salmonella typhi.ĬULTURE CHARACTERISTICS OF SALMONELLA TYPHI (S. ![]() ⇒ The Wilson & Blair bismuth sulfite medium which is an excellent selective medium for the S. Bile salts, Brilliant green dye, Sodium citrate & Sodium thiosulfate which selectively inhibits the growth of many gram-positive bacteria & coliform bacteria and supports the growth of Salmonella & Shigella. contains 4 key components that make it selective for Salmonella & Shigella i.e. ![]() ⇒ Similarly, the Salmonella – Shigella Agar medium which is also a selective medium for Salmonella typhi & other species of salmonella as well as Shigella spp. contains Deoxycholate and Citrate salts in a concentration that inhibits the growth of many gram-positive bacteria & most of the intestinal flora and supports the growth of Salmonella typhi. ⇒ The Deoxycholate Citrate Agar (DCA) medium which is a Selective medium for Salmonella typhi & other Salmonella Species as well as Shigella Spp. MORPHOLOGY AND CULTURE CHARACTERISTICS OF ESCHERICHIA COLI (E.COLI) The Liquid medium (Trypticase Soy Broth, Nutrient broth etc.)Ĭheck out the Morphology & Culture Characteristics of Escherichia coli (E.Selenite F Broth ( Selective medium for Salmonella & Shigella).Tetrathionate broth ( Selective medium).Xylose Lysine Deoxycholate (XLD) medium ( Selective medium for Salmonella & Shigella).Wilson & Blair bismuth sulfite medium ( Selective medium for Salmonella).Salmonella – Shigella Agar medium ( Selective medium for Salmonella & Shigella).Deoxycholate Citrate Agar (DCA) medium ( Selective medium for Salmonella & Shigella).typhi) in the laboratory and most commonly the MacConkey Agar medium, XLD medium, and Selenite F broth medium is used which may vary as per the SOPs of the laboratory, the other media are as follows – ⇒ There are various culture media used for the cultivation of Salmonella typhi (S. grow best in the presence of oxygen and it is also a Facultative anaerobic organism i.e. ⇒ Oxygen requirements – Salmonella typhi (S. Also, the pH requirements vary as per the strain of Salmonella typhi. typhi can survive at 4.1– 9.0 pH but the maximum growth observed around 6.5-7.5 i.e. ⇒ Optimum temperature –The optimum temperature for the cultivation of Salmonella typhi in the laboratory is 37☌. For the isolation from feces, selenite F broth & XLD medium are commonly used. Commonly the NAM & MacConkey Agar medium is used for the cultivation of Salmonella typhi in Laboratory. typhi have no complex nutritional requirements and readily grow in an ordinary media like Nutrient Agar medium (NAM). ⇒ Special requirements – Salmonella typhi or S. MORPHOLOGY OF SALMONELLA TYPHI CULTURE REQUIREMENTS OF SALMONELLA TYPHI (S. Gram Staining Reaction – Salmonella typhi is a Gram -ve (Negative) bacterium. Spores – The Salmonella typhi is a non–sporing bacterium.Ĭapsule – S. Motility – Salmonella typhi is a motile bacterium.įlagella – Salmonella typhi is a flagellated bacterium with peritrichous flagella arrangement. Size – The size of Salmonella typhi is about 1–3 µm × 0.5–0.6 µm (micrometer).Īrrangement Of Cells – Salmonella typhi is arranged singly or in pairs. Shape – Salmonella typhi is a rod shape (bacillus) bacterium. That’s All about the Morphology & Culture Characteristics of Salmonella typhi (S.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |